With Ashley Cole suspended, the Portuguese defender will be out of his comfort zone in the left-back position but he has assured manager Guus Hiddink he can handle the pressure.
Related ArticlesPaper View: Chelsea set for Messi and CoBarcelona v ChelseaDaily Bung: Messi just the leading man in cast of starsCatalans love to hate ChelseaBarca's perfect 10Hiddink turns to Drogba"It is not problem marking Messi," he said. "I'm prepared for the challenge.
"Messi is a big star world wide but it is possible to stop him - with patience and calm. You need to keep him under pressure.
"I have experience playing with Porto and Portugal against big players and now with Chelsea. I do not fear the Argentine."
Bosingwa has received the public backing of manager Guus Hiddink ahead of the first-leg at the Nou Camp, aware the 26-year-old will take on one of the best wingers in the world, the Dutchman said: "I like very much the way he [Messi] plays, he does like the stardom of playing and it's very effective. It looks very simple but it is very difficult.
"But I have full confidence that Bosingwa can do it the job tomorrow. We have some more options, but if he is doing well he can do the job."
Meanwhile, Chelsea winger Florent Malouda believes that the winner would be the side that managed to impose its different style on their opponent.
"I think that everyone enjoys the way that Barca play as they score a lot of goals, where all the players on the pitch work hard and hopefully we can match that," said the Frenchman, who has flourished under Hiddink.
"We have reached the semi-finals, won the right to face Barca and we are both big sides with plenty of ambition," he added.
"Barcelona have a passing game and we also play football but are more physical and so the winner will be the team whose approach triumphs," said Malouda.
"I think Messi is the best player in the world, not many players are capable of what he can do and hopefully we will not see all his qualities. We have all been working hard in training to deal with the threat."
Malouda - part of the France side that surprisingly reached the 2006 World Cup final - has become an important figure for Chelsea in the second half of the season and he can play a crucial role against Barcelona. He puts his improvement down to hard work.
"The English league is very strong and it takes some time to adapt but as well as that I started the season with some injury problems," he said.
"I have been trying hard in training and hopefully my good form has come at the right time," he added.