The defender missed three games after initially sustaining the injury in the 4-0 home win against Atletico Madrid in the Champions League last month.
Related ArticlesDebate: is the title race over?Peter Kenyon goes into showbizChelsea 1 Manchester United 0Chelsea 4 Atl?co Madrid 0Carlo Ancelotti unhappy with United protestsHe returned against Madrid in the return Group D game last week but required a painkilling injection in order to play against United.
A spokesman for Chelsea confirmed: ''Ashley Cole has not been selected in the England squad for their friendly against Brazil.
''He has a depressed fracture of the tibia, an injury first picked up in the Atletico Madrid home match last month.
''He aggravated the injury before the match against Manchester United and required an injection to play.
''However, he further injured it during the game and has therefore been ruled out of this week's England squad.''