Cardiff are waiting to hear the extent of the hamstring injury Ross McCormack suffered in the 1-1 Blackpool draw.McCormack pulled up sharply in the 36th minute and was stretchered off.
"Ross definitely won't play at Plymouth on Tuesday, we heard a ping," said manager Dave Jones, who also saw Paul Quinn and Stephen McPhail get injuries.
Scotland striker McCormack has been the subject of sustained transfer interest from Premier League clubs, but the news could see him staying at the Bluebirds.
Hull are believed to have bid £3.5m for the 22-year-old, Cardiff's top scorer with 23 goals last season.
But their interest cooled when the Bluebirds insisted they would not budge from a £5m valuation for the player who signed a new four-year deal at the Championship club last month.
Should McCormack's problem turn out to be lengthy - hamstring injuries are notoriously problematic for footballers - that could also signal a drop in interest from Premier League suitors.
McCormack signed for Cardiff from Motherwell for £110,000 last summer, but he requested the chance to talk to interested Premier League parties after being told he is not guaranteed a place up front for the Bluebirds.
He played in Scotland's World Cup qualifying defeat by Norway in midweek and now faces a battle to be fit for Scotland's crucial double-header against Macedonia on 5 September and Netherlands four days later.
A serious hamstring injury could rule him out for up to six weeks, meaning he would miss the two games that will decide whether Scotland qualify for the play-offs in second place behind the Dutch.
Cardiff goalkeeper and Scotland international colleague David Marshall said: "Hopefully his hamstring is not too bad and we can get him back shortly because he's one of our best players."
Bluebirds manager Dave Jones says he has been pleased with the way his side has handled a demanding start to the season, but admits that his squad is now down to its bare bones.
"We're putting kids on and players who have been ill all week, it's hard," Jones told BBC Sport Wales.
"Joe [Ledley] looks absolutely drained, he played 45 minutes for us [at Blackpool], 90 for the Wales team [in Montenegro] and he's been ill all week.
"[Chris Burke] did really well considering I've not seen him for a week.
"He trained a bit [Friday] and I had to throw him on [against Blackpool on Saturday]."
With McCormack definitely out of the Plymouth match, Jones will assess the extent of Quinn and McPhail's injuries, along with the knee problem that saw Gabor Gyepes miss the trip to Bloomfield Road.