Cardiff hope Whittingham and Bothroyd are fit as they and Chopra have scored 25 of their 31 league goals this term.
Fit-again striker Ross McCormack is set for his first start for three months.
The Scotland international, Cardiff's top-scorer last season with 23 goals, has not started since tearing his hamstring in Cardiff's 1-1 draw at Blackpool on 15 August.
Stephen McPhail will, however, certainly misses the all-Wales Championship clash after tearing a thigh muscle in Sunday's 1-1 draw with Nottingham Forest.
Jones keeps derby in 'perspective'
The Irish midfielder is out for up to eight weeks and may undergo surgery next week to rectify the problem.
Scotsman Gavin Rae is expected to replace McPhail to partner Joe Ledley in the centre of Cardiff's midfield.
But talismanic striker Bothroyd and form man Whittingham, who has scored nine goals in his last seven games, would be major blows especially due to Chopra's self-inflicted absence after he picked up his fifth booking of the season against Forest.
Bothroyd is a key linkman in Cardiff's attack and a majority of their attacking play goes through the former Arsenal trainee.
Both players have missed training this week and have been undergoing intensive physiotherapy in a bid to be fit for the first Welsh derby of the season.
Swansea have no new injuries and their back-up goalkeeper David Cornell, who has won his first senior Wales call for their forthcoming friendly against Scotland, is returning from Spain to available as Dorus de Vries' reserve in Saturday's derby.
The 18-year-old is flying back on Friday after representing the Wales under-19 side in their back-to-back Uefa Championship qualifying defeats to Portugal on Tuesday and hosts Spain on Thursday.