The Wales international defender was carried off on a stretcher in the second half after a challenge on Wigan's Victor Moses.
Injury blow: Sam Ricketts (right)
The injury cast a shadow over Bolton's 1-0 victory.
Team-mate Kevin Davies tweeted: 'Sam going in for an op on his achilles tomorrow (Friday), will be out for up to 6 months, gutted for him.'
Explore more:People: Kevin Davies, Victor Moses Places: Wales