Billy Davies' Championship outfit Forest will be looking to cause a shock against their Premier League visitors, who won the League Cup in 2002, after seeing off Middlesbrough in round two.
Rovers, though, will know that the competition represents an opportunity for silverware and, after beating Gillingham last time out, manager Sam Allardyce will be eager to record a win.
As is the modern tradition, it is likely both bosses will make changes to their first-team, with league football the priority, and fringe players are set to feature.
Nathan Tyson is likely to remain in a wide role for Forest as the speedy striker has been tormenting defences in the Championship this season as a left winger.
He returned to the team against Blackpool on Saturday in that position after missing two matches due to the birth of his son.
Blackburn-born Joe Garner will return to the Forest squad against his hometown team.
RiskGarner, who began his career with the Lancashire club, was suspended for the defeat to Blackpool but will compete for a striking role along with Dele Adebola, Dexter Blackstock, Robert Earnshaw, David McGoldrick and possibly Tyson.
Paul Smith could replace Lee Camp in goal having played in previous rounds, but Radoslaw Majewski is unlikely to be risked.
It was hoped the Polish midfielder, whose extra-time goal against Middlesbrough got the Reds to this stage of the competition, might return from a knee problem against Blackpool but he suffered a slight setback on the eve of that game.
Blackburn strikers Nikola Kalinic and Benni McCarthy are likely to be given run-outs after only making the bench for the defeat at Everton.
Midfielders Steven Reid, who has only played in the last round of the cup at Gillingham so far this season, Steven Nzonzi and Brett Emerton are in contention.
Defender Gael Givet injured a knee at Goodison Park and will definitely not feature, while on-loan forward Franco Di Santo is unavailable as Chelsea do not want him to be cup-tied.
Defender Lars Jacobsen could also return after he missed the Everton defeat because his wife gave birth on Sunday.