Warnock alleged that Diouf abused Mackie as he awaited a stretcher following a clash with Gael Givet in lhe FA Cup third-round tie at Ewood Park.
Warnock said calling Diouf a sewer rat 'might be insulting to sewer rats' in an attack on the former Liverpool and Bolton player.
Adding insult to injury: Diouf (right) stands over Mackie after the QPR striker's leg was broke
Diouf, 30, has been no stranger to controversy during his time in England but has strenuously denied Warnock's claims.
Diouf told Sky Sports News: 'I am very sorry for the lad to have broken his leg, the QPR player."But what Warnock said - I never said that.
'After the game I argued with some players, that's it. When the lad broke his leg, I never said anything back to him. I never talked to him, you can ask Benjani or Ryan Nelsen. Everything Warnock said is not right.'
Diouf also turned his fire on Warnockhimself, claiming he is not worthy of discussion.
He said: 'I am not disappointed with what Warnock said. Who is Warnock? He is nothing to me. He is not Alex Ferguson, he is not Arsene Wenger, he is not Sam Allardyce or an important manager.'
WHAT THEY SAID ON TWITTER 'Proper disappointed that Diouf was in my ear while aim (I am) on the deck with a broken leg saying **** u and **** your leg thanks' - JAMIE MACKIE @jamiemac12
'That **** will get what's coming to him!!!!' 'Sewer rat pretty much sums that **** right up!!!!' - CLINT HILL @angryhead28
'Never come across a more repulsive human being than E H Diouf! The things he was saying were disgusting! The lad has just broke his leg!' 'You horrible disgusting man E H Diouf! Your time will come!' - BRADLEY ORR @bradleyorr2
Diouf believes he is too often judgedon his past reputation. He said: 'Of course, but I am a strong man and Ican survive with that.'
Blackburn and and QPR players were involved in a tunnel brawl after Diouf's apparent 'sick' foul-mouthed rant at Mackie.
QPR manager Neil Warnock and his players were incensed after seeing Diouf sneer at the stricken Mackie, accusing him of feigning injury when he had suffered a double fracture of his right leg.
Sportsmail revealed that Diouf 's refusal to show any remorse after Saturday's game led to a scuffle in the Ewood Park tunnel.
Mackierevealed on Sunday that, as he lay on the turf after the damaging tackle with Givet, Diouf stood over him and taunted: '**** you and **** your leg'.
Warnock described Diouf as a 'sewer rat' and claimed Blackburn boss Steve Kean wants to sell him, while hardman QPR defender Clint Hill issued Diouf with a warning that he 'will get what's coming to him'.
After Mackie's injury, Diouf became embroiled in a running verbal battle with several Rangers players, and the Senegal striker then aimed a slap at defender Bradley Orr at the final whistle.
The bad blood spilled over in the tunnel when Diouf renewed his attack on Mackie. A witness told Sportsmail: 'QPR striker Rob Hulse went over to Diouf to explain that Mackie had broken his leg and asked whether he would apologise.
'Diouf, in no uncertain terms, said how he didn't care about Mackie's leg and told the QPR players where to go. Then it all kicked off.
'What Diouf was saying was sickening - I can't believe he can say that about a fellow professional who had just broken his leg.'
Warnock claimed to have received an apology from a Blackburn official over Diouf 's behaviour and predicted Kean will seek to rid his dressing room of the controversial forward.
On target: Hoilett scored the only goal of the game as Blackburn progressed to the next round
One senior Blackburn player confided to QPR opponents that Diouf had been a 'disgrace', and a Rovers fan called BBC radio's 606 phone-in to demand the club sack him.
Warnock said: 'One lady from the Blackburn staff came and apologised to me. There's no need for it in the game, I don't know why he does it. You don't need that in the dressing room.
'I think their lads were all the same as the lady. I said to our lot, "Don't get carried away. We only see him once. They've got to live with him".
'Steve Kean will get rid of him, I'll be surprised if he's at Blackburn next year and, really, the sooner he's out of the country the better.
'It's about time he took it to another continent. I don't think he'll play in our game much longer, if I'm honest.'
QPR players used their Twitter pages to vent their anger at Diouf. Orr wrote he had 'never come across a more repulsive human being than Diouf'. And Mackie was equally incensed, describing Diouf's alleged behaviour as 'repulsive'.
The 25-year-old said: 'I'm lying on the floor with a bad injury and even his team-mates were embarrassed by what Diouf was saying.
'There's no room for that in the game - and in my opinion he's an absolute disgrace. I'm trying hard not to think about Diouf. That's because when I do it makes me want to snap my cast off and run up there and find him.
'There's no place in football for the likes of Diouf. There's a line and he crossed it. Diouf was repulsive.'
Kean vows to investigate Diouf's alleged taunts at QPR crock MackieLeo's London: Diouf should not be in the game if Mackie slurs are trueTunnel fight after Diouf's sick jibes! Mackie the target over broken leg
Explore more:People: Alex Ferguson, Bradley Orr, Ryan Nelsen, Neil Warnock, El Hadji Diouf, Sam Allardyce Places: Liverpool, Bolton, United Kingdom