Mark Byrne is ruled out of Barnet's home meeting with Gillingham.
The on-loan Nottingham Forest midfielder came on as a substitute against Northampton at the weekend after recovering from a knee injury.
However, he had to come off again 15 minutes later after injuring his other knee in a collision with Cobblers goalkeeper Chris Dunn.
Rossi Jarvis or Clovis Kamdjo will be added to the squad in Byrne's absence.
Boss Mark Stimson is likely to name an unchanged starting line-up against his former club following Saturday's thumping 4-1 victory which lifted the Bees off the foot of the table.
Defenders Darren Dennehy and Daniel Leach (both foot), winger Glenn Poole (ankle) and striker Danny Kelly (hamstring) are still sidelined.