Two suspended for Ayr
Ayr are without suspended duo Ross McCrorie and Craig Moore for their second-leg clash with Stranraer.
Both players picked up their second caution of the play-offs in Wednesday night's 1-1 draw.
Manager Ian McCall is looking for his players to be composed in their approach, and said: "Things were a bit rushed and frantic in midweek. Some of our players were almost too up for the game and they need to have a calmer approach this time.
"Our injury-time goal has given us momentum but Stranraer played better than us on Wednesday and if that happens again we could miss out on promotion."
Stranraer miss only player-assistant manager Lee Mair (ankle) as they look to get the better of their south-west rivals.
Manager Brian Reid said: "We could focus on the chances we missed and that Ayr scored with virtually the last kick of the ball, but that would not take us forward.
"We will concentrate on how well we played and the fact that if we play that well again then we have a good chance of winning.
"I would have preferred to have had a lead, but with the score at 1-1 we know we need to treat this game as a cup final."