Dutch international goalkeeper Maaten Stekelenburg has been left out of the Roma squad to face Atalanta later on Saturday.
The 29-year-old suffered a worrying head injury in a collision with Inter Milan's Lucio in the second game of the season and has been out ever since.
He was rumoured to be ready to make his comeback but Roma coach Luis Enrique has decided not to risk the former Ajax player.
Also out is Brazilian centre-back Juan.
Atalanta have had the best start of any team this season in Serie A with three wins and a draw in their first four games but are still in the bottom half due to a six-point match-fixing penalty.
Roma earned their first win of the season last week with a 1-0 victory against Parma.