Kevin MacDonald has been the side's caretaker boss since Martin O'Neill's shock departure in the summer.
And the Scot's final game at the helm is to be at Villa Park on Saturday before Houllier takes the reins.
Villa have been waiting for former Liverpool coach Houllier to tie up his exit from his role as the French Football Federation's technical director.
And now Houllier, who has penned a three-year deal at Villa, will make his bow as manager in the Carling Cup clash versus Blackburn Rovers.
"It has been confirmed Gerard Houllier will take charge of the team after the Bolton game," said a club statement.
"He will be in charge for the Carling Cup game against Blackburn Rovers next Wednesday."