The Disney-owned broadcaster will be able to send clips of goals and highlights to mobile phones for the next three seasons, starting in August.
ESPN already screen some live Premier League matches after they took over some of rights from collapsed TV station Setanta.
Access: Fans will soon get Premier League mobile clips from ESPN
Premier League chief executive Richard Scudamore said: 'ESPN's purchase of Barclays Premier League mobile clip rights demonstrates their commitment to the competition and the fans.
'Mobile is an increasingly important technology as hand-held devices become ever more sophisticated, therefore a good offering with a strong partner is important to us both in terms of accessibility and complementing live coverage.'
Lynne Frank, senior vice-president of ESPN in Europe, the Middle East and Africa, said: 'Mobile devices are an indispensable lifeline for football fans, who expect easy, quick access to the latest goals and action wherever they are.
'Adding these rights will allow us to advance our commitment to delivering great sport across the many screens UK fans use each day.'
Sky ordered to slash the current price of its leading sports channels Premier League considering legal challenge to Sky TV rulingCharles Sale: Hull and Wigan to face pitches battle