The Belgium international had to be replaced after just 19 minutes at White Hare Lane and joins fellow first choice centre-half William Gallas on the sidelines.
Injury blow: Crocked Thomas Vermaelen will miss the next two to three weeks
'Certainly it is a calf strain. (He will be out for) two to three weeks,' said Gunners boss Arsene Wenger, speaking to the club's official website.
Vermaelen has been an ever-present for the Gunners since his £10million arrival from Ajax last summer, missing only one Premier League game this season and that was due to suspension, absent during last month's 1-0 win over Wolves.
Sol Campbell and Mikael Silvestre are expected to continue their partnership at the back, with Vermaelen a certainty to miss this weekend's trip to lowly Wigan and the club's Emirates clash against fourth place Manchester City on April 24.
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